Interactive cell analysis

Mayachitra imago's interactive segmentation method segregates the background from the foreground, given the information provided by the user in terms of mouse-drawn scribbles. All you need to do is to provide a few scribbles indicating foreground (e.g. cells or tissue) and background. The segmentation result is updated dynamically as a scribble is added or removed. imago computes the properties of individual cells such as area, perimeter, and average intensity.

Features

Simple to use

imago interactive segmentation method is very intuitive. imago segregates the background from the foreground, given the information provided by you, however, you don't need to guess any parameters. All you need to do is to provide a few scribbles indicating foreground (e.g. cells or tissue) and background.

Flexible refinement

None of the segmentation methods can be perfect. imago provides you flexible editing tools, brush and eraser, to refine the segmentation result.

Save data and images

Keeping an analysis record is important. imago always saves the history of cell analysis results and images. With a single click, the images of cell analysis result can be saved to the hard disk for your publication/presentation.

Easy to search the results

imago's powerful search can help you to find images by the cell analysis results. For example, you can find all images from your album with a cell counter larger than 1000 cells.

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Applications

imago's cell analysis tool can be used for any type of images. It does not need to be limited to any specific stains or imaging technologies. Click the images for segmentation results.

Soybean plant segmentation

Soybean plant.

Astrocyte counting

Astrocytes from a mouse retina.

Muscle cell segmentation

Muscle cells.

Potato cells

Potato tuber cells.

How it works

Cell analysis is done in three quick steps!

Step by step cell analysis tutorial

Step 1. Choose a segmentation method.

You can use either imago's Scribble segmentation method or a simple thresholding method to segment cells from the selected image.

imago's automatic cell counting wizard

Step 2. Apply a segmentation method.

For Scribble segmentation, scribble on the foregorund (e.g.) a cell that you want to segment and the background. Continue to add or remove scribbles for foreground/background until you are satisfied with the result.

Step 3. Refine the result.

You can refine the results using the brush and eraser.

Done.

imago computes the properties of individual cells such as area and average intensity from the segmentation result.

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Video tutorials

It will be coming soon.