Automatic nuclei detector
Mayachitra imago's automatic nuclei detector is an image based powerful cell counter. It automatically identifies nuclei's centroid locations from the selected image and provides you the total number of cells and their location information. imago's nuclei detector is incredibly simple to use yet powerful and robust method to analyze large sets of digital micrographs.
Features
Robust automatic nuclei detector
imago's nuclei detector has been tested on large variety of biological images.
Simple to use
imago's nuclei detector is incredibly easy to use. You can count any type of cells by simply following a three-step cell counting wizard.
Combine a result with manual counting
You can move, add, or remove cell locations with imago's graphical annotation tool. imago also keeps track of the history of modification.
Save data and images
Keeping an analysis record is important. imago always saves the history of counting results and images. With a single click, the images of cell counting result can be saved to the hard disk for your publication/presentation.
Easy to search the results
imago's powerful search can help you to find images by the cell counting results. For example, you can find all images from your album with a cell density larger than 1000 cells/mm2.
Applications
imago's nuclei detector can detect any circular objects from an image. Cell counting does not need to be limited to nuclei stains such as TOPRO or DAPI. Click the images for cell detection results.
Retinal pigment epithelial cells stained with Hoechst (blue).
Embryonic stem cells.
Human subretinal cells stained with TO-PRO (blue).
Photoreceptors stained with TO-PRO. Cells are densely packed.
Amacrine cells in a retina labeled with ChAT.
Fruit fly cells.
Mosaicked image of BrdU labeled mouse retina.
How it works
Cell counting wizard
Cell counting is done in three quick steps!
Step 1. Specify the region to count cells.
You can apply the nuclei detector to either part of an image or the entire image.
Step 2. Choose a cell size.
Simply draw a circle around an example cell on the image. The results are instantly updated in the preview window.
Video tutorials
For more information, watch a video tutorial.
How to apply a nuclei detector to an entire image.
See how counting is done with Mayachitra imago's cell counting wizard.
How to apply a nuclei detector to the part of image.
This video tutorial walks through how to apply imago's nuclei detector to the part of an image.
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