Working with stack images

Browsing a time lapse stack

Double click a time lapse stack that you want to analyze. imago will open it with imago's image viewer. You can browse each image within a time lapse stack by using the horizontal slider. time lapse stack slider

Browsing a z-stack

imago's image viewer will take you to view an individual image easily. You can browse each image by using either the vertical slider or mouse wheel.

Pseudo color visualization

channel selector

Collecting an arbitrary number of channels (e.g. 2 or 4 channels) from a tissue has become a common task. Now you can assign a color to each channel using imago's pseudo-color lookup table. You can display all channels at the same time. If you have a double staining micrograph, imago will automatically display it with a combination of magenta and green, and the image is already ready to submit to publication.

  1. Click a pseudo-color lookup table button next to channel.
  2. Select a color from the lookup table.

Z-projection of a stack

imago makes it easy to create a single image by projecting an image stack along the axis perpendicular to the image plane (the "z" axis). imago creates a projected image by choosing the the maximum value over all images in the stack at the particular pixel location.

Click the blue square button under z-slider. imago will display the projected image in a new window.

Z-projection

Save a z-projection image

The projected image can be stored in the same album by simply clicking Save button. imago also transfers the metadata from the original image to the projection image.

Save a z-projection image

Work with each slice or an entire stack

When it comes to working with stacks, imago offers you flexible tools. You can annotate and measure an interesting part of tissue on a single slice or an entire stack. Annotating and visually analyzing a stack is as easy as a single image.

If you want to count cells from one slice and create a binary image from the other slice, this is no problem for imago. You don't need to remember which analysis function is applied to which slice. imago keeps track of all operations. You can browse all annotations, measurements and analysis results you made by moving the z- or t- sliders. Of course, imago features powerful search tools to help you to find any slice with any changes you made quickly.

Thumbnail overview of your stack images

Mayachitra imago also provides an overview of a stack image even before opening an image. You can see individual images within a stack by moving the mouse pointer either up and down for z-series, or left and right for time series on the thumbnail of a stack image.

Getting started